AHEART February 47/2

نویسندگان

  • MASATO TSUTSUI
  • HISASHI ONOUE
  • YASUHIKO IIDA
  • LESLIE SMITH
  • TIMOTHY O’BRIEN
  • ZVONIMIR S. KATUSIC
  • Hisashi Onoue
  • Yasuhiko Iida
  • Zvonimir S. Katusic
چکیده

Tsutsui, Masato, Hisashi Onoue, Yasuhiko Iida, Leslie Smith, Timothy O’Brien, and Zvonimir S. Katusic. Effects of recombinant eNOS gene expression on reactivity of small cerebral arteries. Am. J. Physiol. Heart Circ. Physiol. 278: H420–H427, 2000.—Resistance arteries are an important target for vascular gene therapy because they play a key role in the regulation of tissue blood flow. The present study was designed to determine the effects of recombinant endothelial (e) nitric oxide synthase (NOS) gene expression on vasomotor reactivity of small brain stem arteries (internal diameter, 253 6 2.5 μm). Arterial rings were exposed ex vivo to an adenoviral vector (109 and 1010 plaque-forming units/ ml) encoding eNOS gene or b-galactosidase gene. Twentyfour hours after transduction, vascular function was examined by isometric force studies. Transgene expression was evident mainly in adventitia. In arteries with endothelium transduced with eNOS gene but not with control b-galactosidase gene, relaxations to bradykinin and substance P were significantly augmented. Removal of endothelium abolished relaxations to bradykinin and substance P in control and b-galactosidase arteries. However, in endothelium-denuded arteries transduced with recombinant eNOS, bradykinin and substance P caused relaxations that were abolished in the presence of the NOS inhibitor NG-nitro-L-arginine methyl ester. In control arteries, endothelium removal augmented relaxations to the nitric oxide donors sodium nitroprusside and diethylamine NONOate. This augmentation was absent in eNOS gene-transduced arteries without endothelium. Our results suggest that, in small brain stem arteries, expression of recombinant eNOS increases biosynthesis of nitric oxide. Adventitia of small arteries is a good target for expression of recombinant eNOS. Genetically engineered adventitial cells may serve as a substitute source of nitric oxide in cerebral arteries with dysfunctional endothelium.

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تاریخ انتشار 2000